Optimization of Biodiesel Production Using Immobilized Candida Rugosa Lipase on Magnetic Fe3O4-Silica Aerogel

Document Type: Research Article

Authors

1 Transport Phenomena Research Center, Faculty of Chemical Engineering, Sahand University of Technology, 51335-1996 Sahand, Tabriz, I. R. IRAN

2 Department of Chemical Engineering, Ahar Branch, Islamic Azad University, 5451116714 Ahar, I. R. IRAN

Abstract

Hydrophobic magnetic silica aerogel was used as a support to immobilize Candida rugosa lipase by adsorption method. Physical and chemical properties of the support and immobilized lipase were determined by Field Emission Scanning Electron Microscope (FESEM), Brunauer–Emmett–Teller (BET) analysis and Fourier Transform InfraRed (FT-IR) spectroscopy and the results showed that the lipase was successfully immobilized onto the support. Biodiesel production from sunflower oil using immobilized lipase was investigated. Response Surface Methodology (RSM) was employed to evaluate the effect of process variables namely methanol/oil molar ratio (4:1-6:1), enzyme concentration (4-10 % mass fraction of oil) and water concentration (3-10 % mass fraction of oil) on biodiesel yield and predict the optimal reaction conditions. A second-order regression model with a high coefficient determination value (R2= 0.99) was fitted to predict the response as a function of reaction parameters. The results indicated that optimum values for methanol/oil molar ratio, enzyme concentration, and water concentration were obtained at 4.5:1, 9.4% and 7.4 %, respectively, in which biodiesel yield was predicted at 72.3%. As the difference between the experimental and predicted values were shown as non-significant, the response surface model employed could be considered as adequate.

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[1] Narwal S.K., Gupta R., Biodiesel Production by Transesterification Using Immobilized Lipase, Biotechnol. Lett., 35: 479-490 (2013).

[2] Feyzi M., Lorestani Zinatizadeh A., Nouri P., Jafari F., Catalytic Performance and Characterization of Promoted K-La/ZSM-5 Nanocatalyst for Biodiesel Production, Iran. J. Chem. Chem. Eng. (IJCCE), 37: 33-44 (2018).

[3] Zarei A., Amin N.A.S., Talebian-Kiakalaieh A., Zain N.A.M., Immobilized Lipase-Catalyzed Transesterification of Jatropha Curcas Oil: Optimization and Modeling, J. Taiwan Inst. Chem. Eng., 45: 444-451 (2014).

[4] Jitputti J., Kitiyanan B., Rangsunvigit P., Bunyakiat K., Attanatho L., Jenvanitpanjakul P., Transesterification of Crude Palm Kernel Oil and Crude Coconut Oil by Different Solid Catalysts,Chem. Eng. J., 116: 61-66 (2006).

[5] Bajaj A., Lohan P., Jha P.N., Mehrotra R., Biodiesel Production Through Lipase Catalyzed Transesterification: An Overview, J. Mol. Catal. B: Enzym., 62: 9-14 (2010).

[6] Amirkhani L., Moghaddas J., Jafarizadeh-Malmiri H., Optimization of Candida rugosa Lipase Immobilization Parameters on Magnetic Silica Aerogel Using Adsorption Method, Iran. J. Chem. Eng. (IJCCE), 13: 19-31 (2016).

[8] Shah S., Gupta M.N., Lipase Catalyzed Preparation of Biodiesel from Jatropha Oil in a Solvent Free System, Process Biochem., 42: 409-414 (2007).

[9] Yagiz F., Kazan D., Akin, A.N., Biodiesel Production from Waste Oils by using Lipase Immobilized on Hydrotalcite and Zeolites, Chem. Eng. J., 134: 262-267 (2007).

[10] Salis A., Pinna M., Monduzzi M., Solinas V., Comparison Among Immobilised Lipases on Macroporous Polypropylene Toward Biodiesel Synthesis, J. Mol. Catal. B: Enzym., 54: 19-26 (2008).

[11] Liu C.-H., Huang C.-C., Wang Y.-W., Lee D.-J., Chang J.-S., Biodiesel Production by Enzymatic Transesterification Catalyzed by Burkholderia Lipase Immobilized on Hydrophobic Magnetic Particles, Appl. Energy 100: 41-46 (2012).

[12] Noureddini H., Gao X., Philkana R., Immobilized Pseudomonas cepacia Lipase for Biodiesel Fuel Production from Soybean Oil, Bioresour. Technol. 96: 769-777 (2005).

[13] Nassreddine S., Karout A., Lorraine Christ M., Pierre A.C., Transesterification of a Vegetal Oil with Methanol Catalyzed by a Silica Fibre Reinforced Aerogel Encapsulated Lipase, Appl. Catal. A: Gen. 344: 70-77 )2008(.