Document Type: Research Article
Department of Chemical Engineering, Tehran Science and Research Branch, Islamic Azad University, Tehran, I.R. IRAN
Department of Nanobiotechnology, Pasteur Institute of Iran, Tehran, I.R. IRAN
Department of Chemical Engineering, South Tehran Branch, Islamic Azad University, Tehran, I.R. IRAN
Aim and Background: Along with efficacy increase of the various drugs, drug side effects have been reduced by nanoparticles. In this study, different nanoparticle formulations of doxorubicin (Doxil) anticancer drugs were prepared. The efficacy of these formulations in the cell culture medium was studied and compared with the free drug.
Materials and Methods: Reverse phase evaporation was used to form the liposome containing Doxil. The graphite nanoparticles were prepared. These nanoparticles were mixed with the liposome containing Doxil and the related nano-complex was conjugated. Spectroscopy methods for visible light-ultraviolet light and light dynamics differentiation were used to describe nanoparticles. For evaluating the toxicity of different formulation, MTT and MCF-7 cells were used.
Results: The amount of drug loading in the liposomes was 72%. The largest amount was related to the nano-conjugated complex and the smallest size was related to the graphene-oxide nanoparticle at a nanometer size. The controlled release in 96 hours and the amount of drug release was 95.43%. Doxil-containing liposome toxicity was 75% and nano- conjugated complex was 85% at the lowest drug concentration (10 µM). The free drug created 35% cell toxicity in 10µM and 89% in 2500 µM.
Conclusion: The results of the study suggested liposomes as a suitable nanoparticle for Doxil. The effect of nanoparticles of graphene oxide was highlighted in this study since in the presence of this nanoparticle in the complex, toxicity was increased significantly.