Polyphenolic Content, Antioxidant Potential and Antimicrobial Activity of Satureja boissieri

Document Type: Research Article


1 Department of Biochemistry, Faculty of Science and Arts, Iğdır University, Iğdır, TURKEY

2 Muş Alparslan University, School of Health, Department of Nursing, Muş, TURKEY

3 Muş Alparslan University, Faculty of Education, Department of Primary Education, Muş, TURKEY

4 Iğdır University, Health Services Vocational School, Department of Medical Services and Techniques, Iğdır, TURKEY

5 Dicle University, Faculty of Pharmacy, Department of Biochemistry, Diyarbakır, TURKEY

6 Adıyaman University, Pharmacy Faculty, Department of Pharmaceutical Professional Sciences, Adıyaman, TURKEY


Antioxidant activities of Satureja boissieri extracts were detected by using specific in vitro techniques. Standard antioxidant compounds such as ascorbic acid, BHA (Butylated Hydroxyanisole) and BHT (Butylated Hydroxytoluene) were used to compare with the results obtained from ethanol and water extracts of the plant samples. Both extracts presented high antioxidant actions on ABTS cation radical and DPPH free radical scavenging methods. Furthermore, the antimicrobial activity of S. boissieri was defined by using three fungi species, three Gram-positive and four Gram-negative bacteria species. The reducing power antioxidant activities of samples were measured by CUPRAC and FRAP techniques. Also, phenolic compounds of S. boissieri leaves were identified by UHPLC-ESI-MS/MS. The high concentration of hesperidin (5051 ± 247 ppb) and rosmarinic acid (4364 ± 214 ppb) was characterized quantitatively. According to the results, high phenolic content can be thought of as one of the responsible parameters for effective biological activity. Also, its flavonoid and phenolic contents are good natural sources for using in the food industry and pharmacological process.


Main Subjects

[1] Rechinger K.H., Satureja. “Flora Desiranischen Hoclandes and der Umrahmenden Gebirge”, vol. 150, Akademische Druku Verlags Antalt Graz, Austria (1982).

[2] Kilic O., Bagci E., Essential Oil Composition of Wiedemannia Fisch. and C.A.Mey. Genus from Turkey, a Chemotaxonomic Approach, J Essent Oil Bear Pl, 17(5): 741–746 (2014).

[3] Hajhashemi V., Sadraei H., Ghannadi A.R., Mohseni M., Antispasmodicanti-diarrhoeal Effect of Satureja Hortensis L. Essential Oil, J Ethnopharmacol, 71: 187–192 (2000).

[4] Kurkcuoglu M., Tumen G., Baser K.H.C., Essential Oil Constituents of Satureja Boissieri from Turkey, Chem Nat Compd, 37(4): 327–331 (2001).

[5] Azaz D., Demirci F., Satil F., Kurkcuoglu M., Baser K.H.C., Antimicrobial Activity of Some Satureja Essential Oils, Z Naturforsch C, 57(9-10): 817–821 (2002).

[7] Aras A., Bursal E., Dogru M., UHPLC-ESI-MS/MS Analyses for Quantification of Phenolic Compounds of Nepeta Nuda Subsp. Lydiae, J. Ap. Pharma. Sci., 6(11): 9–13 (2016).

[8] Sisecioglu M., Cankaya M., Gulcin I., Ozdemir H., Interactions of Melatonin and Serotonin to Lactoperoxidase Enzyme, J. Enzyme Inhib. Med. Chem., 25: 779–783 (2010).

[9] Yang B., Feng X., Xu J., Lei H., Zhang L., Multi-Component HPLC Analysis and Antioxidant Activity Characterization of Extracts from Dipsacus Sativus (Linn.) Honck, Int. J. Food. Prop., 19(5): 1000–1006 (2016).

[10] Davis P.H., “Flora of Turkey and the East Aegean Islands”, Un. Press, Edinburgh 7: Pp 321 (1982).

[11] Aras A., Doğru M., Bursal E., Determination of Antioxidant Potential of Nepeta Nuda Subsp. Lydiae, Ana. Chem. Lett., 6(6): 758–765 (2016).

[12] Koksal E., Tohma H., Kilic O, Alan Y., Aras A., Gulcin I, Bursal E., Assessment of Antioxidant and Antimicrobial Activities of Nepeta Trachonitica - Analysis of its Phenolic Compounds Using HPLC-MS/MS. Sci .Pharm., 85: 24 (2017).

[13] Oyaizu M., Studies on Product of Browning Reaction Prepared from Glucose Amine, Japan. J. Nutr.,  44: 307–315 (1986).

[15] Tohma H., Koksal E., Kilic O., Alan Y., Yilmaz MA., Gulcin I., Bursal E., Alwasel S., RP-HPLC/MS/MS Analysis of the Phenolic Compounds, Antioxidant and Antimicrobial Activities of Salvia L. Species. Antioxidants,5(4): 38 (2016).

[16] Ertas A., Boga M., Yılmaz M.A., Yesil Y., Tel G., Temel H., Hasimi N., Gazioglu I., Ozturk M., Ugurlu P., A Detailed Study on the Chemical and Biological Profiles of Essential Oil and Methanol Extract of Thymus Nummularius (Anzer tea): Rosmarinic Acid, Ind. Crop. Prod., 67: 336–345 (2015).

[17] Bursal E., Kinetic Properties of Peroxidase Enzyme from Chard (Beta Vulgaris Subspecies Cicla), Int. J. Food. Prop., 16(6): 1293-1303 (2013).

[18] Turrens JF., Superoxide Production by the Mitochondrial Respiratory Chain, Bioscience Rep., 17: 3–8 (1997).

[19] Li W., Beta B., Evaluation of Antioxidant Capacity and Aroma Quality of Anthograin Liqueur, Food Chem., 127: 968–975 (2011).

[20] Abideen Z., Qasim M., Rasheed A., Adnan M.Y., Gul B., Khan M.A., Antioxidant Activity and Polyphenolic Content of Phragmites Karka Under Saline Conditions, Pakistan Pak. J. Bot., 47: 813-818 (2015).

[21] Jacob JK, Hakimuddin F, Paliyath G and Fisher H., Antioxidant and Antiproliferative Activity of Polyphenols in Novel High-Polyphenol Grape Lines, Food Res. Int., 41: 419–428 (2008).

[22] Lopez-Cobo A., Gomez-Caravaca AM., Svarc-Gajic J., Segura-Carretero A., Fernandez-Gutierrez A., Determination of Phenolic Compounds and Antioxidant Activity of a Mediterranean Plant: The Case of Satureja Montana subsp. Kitaibelii, J. Funct. Foods, 18: 1167–1178 (2015).

[24] Hu W., Yu L., Wang MH., Antioxidant and Antiproliferative Properties of Water Extract from Mahonia Bealei (Fort.) Carr. Leaves, Food Chem. Toxicol., 49:799–806 (2011).

[25] Dorman HJD., Peltoketo A, Hiltunen R, Tikkanen MJ., Characterization of the Antioxidant Properties of De-odourised Aqueous Extracts from Selected Lamiaceae Herbs, Food. Chem., 83: 255–262 (2003).

[27] Del Monte D., De Martino L., Marandino A., Fratianni F., Nazzaro F., De Feo V., Phenolic Content, Antimicrobial and Antioxidant Activities of Hypericum Perfoliatum L, Ind. Crop. Prod., 74: 342–347 (2015).

[29] Isik M., Korkmaz M., Bursal E., Gulcin I., Koksal E., Tohma H., Determination of Antioxidant Properties of Gypsophila Bitlisensis Bark, Int. J. Pharm., 11(4): 366–371 (2015).

[30] Sandin-Espana P., Mateo-Miranda M., Lopez-Goti C., De Cal A., Alonso-Prados JL., Development of a Rapid and Direct Method for the Determination of Organic Acids in Peach Fruit Using LC–ESI-MS. Food Chem., 192: 268–273 (2016).

[31] Fiorucci SB., Golebiowski J., Cabrol-Bass D., Antonczak S., DFT Study of Quercetin Activated forms Involved in Antiradical, Antioxidant and Prooxidant Biological Processes, J. Agric. Food Chem, 55, 903–911 (2007).

[32] Devi KP., Rajavel T., Nabavi SF., Setzer WN., Ahmadi A., Mansouri K., Nabavi SM., Hesperidin: A Promising Anticancer Agent from Nature, Ind. Crop. Prod., 76: 582–589 (2015).

[33] Gulcin I., Scozzafava A., Supuran C.T., Koksal Z., Turkan F., Cetinkaya S., Bingol Z., Huyut Z., Alwasel S., Rosmarinic Acid Inhibits Some Metabolic Enzymes Including Glutathione S-Transferase, Lactoperoxidase, Acetylcholinesterase, Butyrylcholinesterase and Carbonic Anhydrase Isoenzymes, J. Enzyme Inhib. Med. Chem., 31(6): 1698–1702 (2016).

[34] Chtourou Y., Slima A.B., Makni M., Gdoura R., Fetoui H., Naringenin Protects Cardiac Hypercholesterolemia-Induced Oxidative Stress and Subsequent Necroptosis in Rats, Pharmacol. Rep., 67: 1090–1097 (2015).

[35] Garro Galvez., JM, Ried B., Conner A.H., Analytical Studies on Tara Tannins, Holzforschung, 51: 235–243 (1997).