Determination of Fatty Acids, α-Tocopherol, β-Caroten, Minerals, and Some Pomological Properties of Walnut Genotypes Selected from Aras Valley (Eastern Turkey)

Document Type: Research Article

Authors

1 Department of Horticulture, Faculty of Agriculture, Igdir University, 76000, TURKEY

2 Department of Horticulture, Faculty of Agriculture, Dicle University, 21280, TURKEY

Abstract

Fatty Acids, α-tocopherol, β-caroten, minerals, and some pomological properties of walnut genotypes from four locations (Iğdır, Tuzluca, Karakoyunlu, Kağızman) of Aras Valley (Eastern Turkey) were examined. In this study, the differences among the genotypes were determined in terms of some fruit characteristics, oil and protein contents, fatty acid composition, selenium content, α-tocopherol content, β-carotene content and some macro- and microelement contents. The genotypes different in the values of nut weight from 8.89 to16.22 g, kernel weight from 4.72 to 9.64 g, kernel ratio from 36.74 to 59.59%, and shell thickness 1.04-3.60 mm, respectively. The contents of fat and moisture of the selected genotypes were in the range of 59.18 to 68.12% and 10.49 to 23.31%, respectively. The contents of most common fatty acids determined in the genotypes were linoleic acid (58.15-64.07%), oleic acid (12.93-17.49%), linolenic acid (9.37-13.61%), palmitic acid (5.60-8.62%) and stearic acid (4.68-6.69%), whereas the contents of remaining fatty acids were rarely detected in trace amounts. In the genotypes,
the amount of α-tocopherol was in the range of 8.75 to 35.11 mg/kg, β-carotene was in the range of 0.03 to 0.12 mg /kg and selenium was in the range of 15.89 to 68.19 ng/g. The  genotypes
were found to have  1.09 to 2.47% N,  230.36 to 451.48 mg/100g P, 350.74 to 666.20 mg/100g K, 
2.30 to 3.86 mg/100g Cu,  1.71 to 3.91 mg/100g Zn,  7.16 to 18.82 mg/100g Fe,  144.0 to 452.08 mg/100g Ca, 110.25 to 342.44 mg/100g Mg,  1.73 to 9.67 mg/100g Mn and  7.11 to 25.51 mg/100g Na. The present results revealed that the high nutritional values of walnut genotypes selected from the Aras Valley(Eastern Turkey) could have health benefits effects on human nutrition.

Keywords

Main Subjects


[1] Şen S.M., “Ceviz Yetiştiriciliği”, Eser Matbaası, Samsun (1986).

[2] Çiftçi K., İzmir ve Manisa İllerinde Ceviz Yetiştiriciliğinin Sosyo-Ekonomik Yönü ve Sorunları Üzerine Bir Araştırma, Ege Üniversitesi Fen Bilimleri Enstitüsü. Yüksek Lisans Tezi. 89 s. İzmir (2004).

[3] Cosmulescu S., Bacıu A., Achim G., Botu M. Trandafır I., Mineral Composition of Fruits in Different Walnut (Juglans regia L.) Cultivars, Not. Bot. Hort. Agrobot. Cluj., 37 (2): 156-160 (2009).

[4] Tapsell L., Batterham M., Tan S.Y., Warensjö E., The Effect of a Calorie Controlled Diet Containing Walnuts on Substrate Oxidation During 8-Hours in a Room Calorimeter. Journal of the American College of Nutrition, 28(5): 611-617 (2009).

[5] Şen S.M., Karadeniz T., The Nutritional Value of Walnut, Journal of Hygienic Engineering and Design, 11: 68-71 (2015).

[6] Reiter R.J., Manchester L.C., Tan D.X., Melatonin iİn Walnuts: Influence on Levels of Melatonin and Total Antioxidant Capacity of Blood, Nutrition, 21(9): 920-924 (2005).

[7] Carey A.N., Poulose S.M., Shukitt-Hale B., The Beneficial Effects of Tree Nuts on the Aging Brain, Nutrition and Aging, 1(1): 55-67 (2012).

[8] Amaral J.S., Alves M.R., Seabra R.M., Oliveira B P., Vitamin E Composition of Walnuts (Juglans regia L.): A 3-Year Comparative Study of Different Cultivars, Journal of Agricultural and Food Chemistry, 53(13): 5467-5472 (2005).

[9] Davis L., Stonehouse W., Loots D.T., Mukuddem-Petersen J., Van der Westhuizen F., Hanekom S.J., Jerling J.C., The Effects of High Walnut and Cashew Nut Diets on the Antioxidant Status of Subjects with Metabolic Syndrome, European Journal of Nutrition, 46: 155-164(2007).

[10] Lavedrine F., Ravel A., VilletA., Ducros V., Alary J., Mineral Composition of Two Walnut Cultivars Originating in France and California, Food Chemistry, 68(3): 347-351 (2000).

[12] Orhan I.E., Suntar I.P., Akkol, E.K., In vitro Neuroprotective Effects of the Leaf and Fruit Extracts of Juglans regia L.(walnut) through Enzymes Linked to Alzheimer's Disease and Antioxidant Activity. International Journal of Food Sciences and Nutrition, 62(8):  781-786 (2011).

[15] AOAC., Official Methods of Analysis, 15th AOAC International, Washington, DC (1990).

[16] James C.S., Analytical Chemistry of Foods. Chapman and Hall.1st ed. London (1995).

[18] Manz U., Philipp K., Method for the Routine Determination of Tochopherols in Animal Feed and Human Foodstuffs with the Aid of HPLC, International Journal for Vitamin and Nutrition Research, 51: 342–348 (1981).

[19] Eittenmiller R.R., Lin Ye W.O., Landen Jr., “Vitamin Analysis for Food the Health and Food Sciences”, CRC Press, New York, Second Edition, 119-191 (2008).

[20] Konings E.J.M., Roomans, H.H.S., Evaluation and Validation of an LC Method for the Analysis of Carotenoids in Vegetables and Fruit, Food Chemistry, 59(3): 599-603 (1997).

[21] Cubbada F., Inductively Coupled Plasma-Mass Spectrometry for the Determination of Elements and Elemental Species in Food: A Review, Journal of AOAC International, 87: 173-204 (2004).

[22] Şimşek M., Osmanoğlu A., Mazıdağı (Mardin) Yöresindeki Doğal Cevizlerin (Juglans regia L.) Seleksiyonu, YYÜ Tar Bil Derg (YYU J Agr Scı) 20(2): 131-137 (2010).

[23] Ali M., Ullah A., Ullah H., Khan F., Ibrahim S.M., Ali L., Ahmad S., Fruit Properties and Nutritional Composition of Some Walnut Cultivars Grown in Pakistan, Pakistan Journal of Nutrition, 9(3): 240-244 (2010).

[24] Simsek M., Chemical, mineral, and fatty acid compositions of various types of walnut (Juglans regia L.) in Turkey, Bulgarian Chemical Communications, 48(1): 66-70 (2016).

[25] Bada J.C., León-Camacho M., Prieto M., Copovi P., Alonso L., Characterization of Walnut Oils (Juglans regia L.) from Asturias, Spain, Journal of the American Oil Chemists' Society, 87(12): 1469-1474 (2010).

[26] Gharibzahedi S.M.T., Mousavi S.M., Hamedi M., Khodaiyan F., Determination and Characterization of Kernel Biochemical Composition and Functional Compounds of Persian Walnut Oil, Journal of Food Science and Technology, 51(1): 34-42.(2014).

[27] Rabrenovic B., Dimic E., Maksimovic M., Sobajic S., Gajic- Krstajic L., Determination of Fatty Acid and Tocopherol Compositions and the Oxidative Stability of Walnut (Juglans regia L.) Cultivars Grown in Serbia, Czech Journal of Food Sciences, 29(1): 74-78 (2011).

[28] Tsao L.R.; Yang, R.; Kramer, J.K. Hernandez M., Fatty Acid Profiles, Tocopherol Contents, and Antioxidant Activities of Hearnut (Juglans ailanthifo var. Cordiformis) and Persian Walnut (Juglans regia), Journal Agric Food Chem., 55(4):   -    (2007).

[29] Özcan M.M., Some Nutritional Characteristics of Fruit and Oil of Walnut (Juglans regia L.) Growing in Turkey, Iran. J. Chem. Chem. Eng. (IJCCE), 28(1): 57-63 (2009).

[30] Ozrenk K., Javidipour I., Yarilgac T., Balta F., Gündoğdu M., Fatty Acids, Tocopherols, Selenium and Total Carotene of Pistachios (P. vera L.) from Diyarbakır (Southestern Turkey) and Walnuts
(J. regia L.) from Erzincan (Eastern Turkey),
Revista de Agaroquimica y Tecnologia de Alimentos, 18(1): 55-62 (2012).

[31] Pereira J.A., Oliveira I., Sousa A., Ferreira I.C., Bento A., Estevinho L., Bioactive Properties and Chemical Composition of Six Walnut (Juglans regia L.) Cultivars, Food and Chemical Toxicology, 46(6): 2103-2111 (2008).

[32] Tinggi U., Selenium: Its Role as Antioxidant in Human Health, Environ Health Prev Med, 13: 102–108 (2008).

[33] Demirel S., Tuzen M., Saracoglu S., Soylak M., Evaluation of Various Digestion Procedures for Trace Element Contents of Some Food Materials, Journal of Hazardous Materials, 152(3): 1020–1026 (2008).

[34] Pappa E.C., Pappas A.C., Surai P.F., Selenium Content in Selected Foods from the Greek Market and Estimation of the Daily İntake, Science of the Total Environment, 372(1): 100–108 (2006).

[35] Khatib S., Vaya J., Fig, Carob, Pistachio and Health, In: Watson R and Preedy VR (eds) “Bioactive Foods in Promoting Health: Fruits and Vegetables”, Oxford: Elsevier Academic Press, 245–263 (2010).

[36] Bai C., Twyman R.M., Farré G., Sanahuja G., Christou P., Capell T., Zhu C., A Golden era—Pro-Vitamin A Enhancement in Diverse Crops, In Vitro Cell. Dev. Biol. Plant, 47: 205–221 (2011).

[37] Berman J., Zorrilla-López U., Farré G., Zhu C., Sandmann G., Twyman R.M., Capell T., Christou P. Nutritionally Important Carotenoids as Consumer Products, Phytochem. Rev., 14: 727–743 (2015).

[38] Kornsteiner M., Wagner K.H., Elmadfa I., Tocopherols and Total Phenolics in 10 Different nut Types, Food Chemistry, 98(2): 381-387 (2006).

[39] Abdallah I.B., Tlili N., Martinez-Force E., Rubio A.G.P., Perez-Camino M.C., Albouchi A., Boukhchina S., Content of Carotenoids, Tocopherols, Sterols, Triterpenic and Aliphatic Alcohols, and Volatile Compounds in Six Walnuts (Juglans regia L.) Varieties, Food Chemistry, 173: 972-978 (2015).

[40] Stuetz W., Schlörmann W., Glei M., B-Vitamins, Carotenoids and α-/γ-Tocopherol in Raw and Roasted Nuts, Food Chemistry, 221: 222-227 (2017).

[41] Kodad O., Estopañán G., Juan T., Sindic M., Genotype and Year Variability of the Chemical Composition of Walnut Oil of Moroccan Seedlings from the High Atlas Mountains, Grasas y Aceites, 67(1): 116-   (2016).

[42] Zhai M., Wang Z., Wang D., Xu J., Shı G., Comparative Analysis of Mineral Elements and Essential Amino Acids Compositions in Juglans sigillata and J. Regia Walnuts, Kernels Not Bot Horti Agrobo, 42(1): 36-42 (2014).

[43] Tapia M.I., Sanchez-Margado J.R., Garcia-Parra J., Ramirez R., Hernandez T., Gonzales-Games D., Comparative Study of the Nutritional and Bioactive Compounds Content of Four Walnut (Juglans regia l.) Cultivars, Journal of Food Composition and Analysis, 31: 232-237 (2013).

[44] Muradoglu F., Oguz H.I., Yildiz K., Some Chemical Composition of Walnut (Juglans regia L.)Selections from Eastern Turkey, African Journal of Agricultural Research, 5(17): 2379-2385 (2010).