Vortex-Assisted Inverted Dispersive Liquid-Liquid Microextraction of Naproxen from Human Plasma and Its Determination by High-Performance Liquid Chromatography

Document Type: Research Article

Authors

Department of Chemistry, University of Mazandaran, Babolsar, I.R. IRAN

Abstract

Extraction and determination of naproxen from human plasma were performed using Vortex-Assisted Inverted Dispersive Liquid-Liquid MicroExtraction (VA−IDLLME) and High-Performance Liquid Chromatography (HPLC). The parameters affecting extraction recovery such as type and volume of extraction and disperser solvents, pH of sample solution, stirring rate, extraction time and salt addition were optimized. Optimal extraction conditions were: 100 µL dodecane as the extraction solvent, 350 µL methanol as disperser solvent, pH of sample = 5, stirring rate: 500 rpm, with no effect of extraction time and no addition of NaCl to the sample solution. Under the optimal conditions a linear range of 1.0–1000.0 ng/mL(R2 = 0.9993) was obtained. Limit of detection, the extraction recovery, and preconcentration factor were 0.32 ng/mL, 87% and 108 respectively.

Keywords

Main Subjects


]1] Todd P.A., & Clissold S.P., Naproxen. Drugs, 40(1): 91-137 (1990).

[2] Boynton C.S., Dick C.F., Mayor G.H., NSAIDs: An Overview, The Journal of Clinical Pharmacology, 28(6): 512-517 (1988).

[3] Trelle S., Reichenbach S., Wandel S., Hildebrand P., Tschannen B., Villiger P.M., Jüni P., Cardiovascular Safety of Non-Steroidal Anti-Inflammatory Drugs: Network Meta-Analysis. Bmj, 342 (2011).

[5] Warner-Schmidt J.L., Vanover K.E., Chen E.Y., Marshall J.J., Greengard P., Anti-Depressant Effects of Selective Serotonin Reuptake Inhibitors (SSRIs) are Attenuated by Anti-Inflammatory Drugs in Mice and Humans, Biological Psychiatry, 68: 528-535 (2010).

[6] Bebawy L.I., El-Kousy N.M., Simultaneous Determination of Some Multicomponent Dosage Forms by Quantitative Thin Layer Chromatography Densitometric Method, Journal of Pharmaceutical and Biomedical Analysis, 20(4): 663-670 (1999).

[7] Kayiran S., Pehlivan S.B., Çelebier M., Ünlu N., Determination of Naproxen Sodium from Poly (Lactide-co-Glycolide) Corneal Scaffolds, Turkish Journal of Pharmaceutical Sciences, 7(1): 57-68 (2010).

[8] Hsu Y.H., Liou Y.B., Lee J.A., Chen C.Y., Wu A.B., Assay of Naproxen by High‐Performance Liquid Chromatography and Identification of Its Photoproducts by LC‐ESI MS, Biomedical Chromatography, 20(8): 787-793 (2006).

[10] Aresta A., Palmisano F., Zambonin C.G., Determination of Naproxen in Human Urine by Solid-Phase Microextraction Coupled to Liquid Chromatography, Journal of Pharmaceutical and Biomedical Analysis, 39(3): 643-647 (2005).

[11] Pedersen‐Bjergaard S., Rasmussen K.E., Liquid Phase Microextraction and Capillary Electrophoresis of Acidic Drugs, Electrophoresis, 21(3): 579-585 (2000).

[12] Mallett D.N., Dayal S., Dear G.J., Pateman A.J., The Determination of Naproxen in Plasma by Mixed-Mode Liquid Chromatography–Tandem Mass Spectrometry Following Ion Exchange Solid Phase Microextraction, Journal of Pharmaceutical and Biomedical Analysis, 41: 510-516 (2006).

[13] Mehta P., Sharma C.S., Nikam D., Ranawat M.S., Development and Validation of Related Substances Method by HPLC for Analysis of Naproxen in Naproxen Tablet Formulations, International Journal of Pharmaceutical Sciences and Drug Research; 4(1): 63-69 (2012).

[15] Farajzadeh M.A., Seyedi S.E., Shalamzari M.S., Bamorowat M., Dispersive Liquid-Liquid Microextraction Using Extraction Solvent Lighter Than Water, Journal of Separation Science, 32: 3191-3200 (2009).

[16] SarafrazYazdi A., Es’haghi Z., Surfactant Enhanced Liquid-Phase Microextraction of Basic Drugs of Abuse in Hair Combined with High Performance Liquid Chromatography, Journal of Chromatography A., 1094: 1-8 (2005).

[20] Ebrahimzadeh H., Yamini Y., Abedi Firozjaei H., Kamarei F., Tavassoli N., Tavassoli N., Rouini M.R., Hollow Fiber-Based Liquid Phase Microextraction Combined Withhigh-Performance Liquid Chromatography for the Analysis of Gabapentin in Biological Samples, J. Analytica Chim Acta, 665: 221-226 (2010).

[21] Van Der Merwe H.M., De Kock M.J., Reversed-Phase High-Performance Liquid Chromatographic Method for the Determination of Warfarin from Biological Fluids in the Low Nanogram Range, Journal of Chromatography B: Biomedical Sciences and Applications, 378: 254-260 (1986).