Removal of Phenols with Encapsulated Horseradish Peroxidase in Calcium Alginate

Document Type: Research Article

Authors

1 Department of Chemical and Petroleum Engineering, Sharif University of Technology,P.O. Box 11365-6841 Tehran, Tehran, I.R. IRAN

2 Department of Chemical and Petroleum Engineering, Sharif University of Technology, P.O. Box 11365-6841 Tehran, Tehran, I.R. IRAN

Abstract

Horseradish peroxidase was encapsulated in calcium alginate for the purpose of phenol removal. Considering enzyme encapsulation efficiency, retention activity and enzyme leakage of the capsules, the best gelation condition was found to be 1 % w/v of sodium alginate solution and 5.5 % w/v of calcium chloride hexahydrate. Upon immobilization, pH profile of enzyme activity changes as it shows higher value at basic and acidic solution. Besides, for each phenol concentration there would be an enzyme concentration which going further than this value has no significant effect on phenol removal. Investigation into time course of phenol removal for both encapsulated and free enzyme showed that encapsulated enzyme had nearly similar efficiency in comparison with the same concentration of free enzyme; however the capsules were reusable up to four cycles without any changes in their efficiency. The ratio of hydrogen peroxide/phenol at which highest phenol removal obtained, found to be dependent on initial phenol concentration and in the solution of 2 and 8 mM phenol it were 1.15 and 0.94 respectively.

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[1] Stanisavljevic, M., Nedic, L., Removal of Phenol from Industrial Wastewater by HRP, Workinh and Living Environmental Protection, 2, 345 (2004).

[2] Ulson de Souza, S.M. Forgiarini, E. Ulson de Souza, A., Toxicity of Textile Dyes and Their Degradation by Enzyme Horseradish Peroxidase (HRP), J. Hazardous Materials147, 1073 (2007).

[3] Bodalo, A., Gomez, J.L., Gomez, E., Hidalgo, A.M., Yelo, A.M., Removal of 4-chlorophenol by Soybean Peroxidase and Hydrogen Peroxidade in a Discontinuous Tank Reactor, Desalination, 147, 1073 (2007).

[4] Singh, N., Singh, J., An Enzymatic Method for Removal of Phenol from Industrial Effluent, Prep Biochem Biotechnol, 32, 127 (2002).

[5] Zhang, J.Y.P., Chen, S., Wang, W., Removal of Pentachlorophenol by Immobilized Horseradish Peroxidase,Int.BiodeteriorationandBiodegradation, 59, 307 (2007).

[6] Miland, E., Malcolm, R.S., Ciaran, O.F., Phenol Removal with Modified Peroxidases, J. Chem. Tech. Biotechnol., 67, 227 (1996).

[7] Aitken, M.D., Wastewater Treatment Applications of Enzymes: Opportunity and Obstacles, Chem. Eng. J. Biochem. Eng. J., 52, B49 (1993).

[8] Wilberg, K., Assenhaimer, C., Rubio, J., Removal of Aqueous Phenol Catalyzed by a Low Purity Soybean Peroxidase, J. Chem. Technol. Biotechnol., 77, 851 (2002).

[9] Fernandes, K.f., Lima, C.S., Lopez, F.M., Collins, C.H., Perarerties of Horseradish Peroxidase Immobilized Onto Polyaniline, Process Biochem., 39, 957 (2004).

[10] Welinder, K.G., Larsen, Y.B., Covalent Structure of Soybean Seed Coat Peroxidase, Biochimica et Biophysica Acta, 1698, 121 (2004).

[11] Lai, Y.C., Lin, S.C., Application of Immobilized Horseradish Peroxidase for the Removal of
p-chlorophenol from Aqueous Solution, Process Biochem., 40, 1167 (2005).

[12] Azevedo, A. M., Vojinovic, V.,  Cabral, J. M. S., Gibson, T.D., Fonseca, L.P., Operational Stability of Immobilized Horseradish Peroxidase in Mini-Packed Bed Bioreactors, J. Mol. Catal., B:Enzyme, 28, 121 (2004).

[13] Fernandes, K.F., Lima, C.S., Pinho, H., Collins, C.H., Immobilization of Horseradish Peroxidase Onto Polyaniline Polymers, Process Biochem, 38, 1379 (2003).

[14] Cheng, J., Yu, S.M., Zuo, P., Horseradish Peroxidase Immobilized on Aluminum-Pillared Interlayered Clay for the Catalytic Oxidation of Phenolic Wastewater, Water Res, 40, 283 (2006).

[15] Caromori,  S. S.,  Fernandes,  K. F.,  Covalent Immobilization of Horseradish Peroxidase Onto Poly(ethylene terephetalate)- Poly (aniline) composite, Process Biochem., 39, 883 (2004).

[16] Lai, Y.C., Lin, S.C., Application of Immobilized Horseradish Peroxidase for the Removal of
p-chlorophenol from Aqueous Solution, Process Biochem., 40, 1167 (2005).

[17] Nakamoto, S., Machida, N., Phenol Removal from Aqueous Solution by Peroxidase-Catalyzed Reaction Using Additives, Water Res., 26, 49  (1992).

[18] Kinsely, C., Nicell, j.A., Treatment of Aqueous Phenol with Soybean Peroxidase in the Presence of Polyethylene Glycol, Biores Tech., 73, 139 (2000).

[19] Nigma, S.C., Tsao, I. F., Sakoda, A., Wang, H.Y., Techniques for Preparing Hydrogel Membrane Capsule, Biotechnol Tech., 2, 271  (1988).

[20] Peterson, G.L., A Simplification of the Protein Assay Method of Lowry et al. that is More Generally Applicable, Anal Biochem., 83, 346 (1977).

[21] Nicell, J.A., Wright, H., A Model Peroxidase Activity with Inhibition by Hydrogen Peroxide, Enzyme Microb. Technol., 21, 302 (1997).

[22] Wu, Y., Taylor,, K.E., Biswas, N., Bewtra, J.K., Comparison of Additive in the Removal of Phenolic Compound by Peroxidase-Catalyzed Polymerization, Water Res., 31, 2699 (1997).

[23] Wu, Y., Taylor, K.E., Biswas, N., Bewtra, J.K., Kinetic Model for Removal of Phenol by Horseradish Peroxidase with PEG, J. Environ. Eng., 125, 451 (1999).

[24] Nicell, J.A., Bewtra, J.K., Biswas, N., Pierre, C. S., Enzyme Catalyzed Polymerization and Precipitation of Aromatic Compound from Wastewater, Can. J. Civ. Eng., 20, 725 (1993).