Preparation and Characterisation of Immunoadsorbents for High Performance Liquid Affinity Chromatography (HPLAC)

Document Type: Research Article

Authors

1 Biotechnology Group, Department of Chemical Engineering, Tarbiat Modares University, P.O. Box 14155-4838 Tehran, I.R. IRAN

2 Department of Chemical Engineering, University of Birmingham, P.O. Box 363, Birmingham, B15 2TT, ENGLAND

Abstract

The efficacy of activation methods and coupling were studied in the context of performance in batch and fixed bed binding experiments utilizing cell culture fluids or blood plasma as feedstock. Conclusions were drawn regarding selection of solid phase according to pore size, rigidity, pH stability, Chemistry of derivation and activation, and gross concentration of immobilized ligand required for optimal performance in analytical and preparative fractionation of complex feedstreams.

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