TY - JOUR ID - 7795 TI - UV-HPLC / APCI-MS Method for Separation and Identification of the Carotenoids Produced by Sporobolomyces ruberrimus H110 JO - Iranian Journal of Chemistry and Chemical Engineering JA - IJCCE LA - en SN - 1021-9986 AU - Razavi, Sayed Hadi AU - Blanchard, Fabrice AU - Marc, Ivan AD - Department of Food Science & Engineering, Faculty of Biosystem Engineering, University of Tehran, Karaj, I.R. IRAN AD - Laboratoire des Science du Génie Chimique, U.P.R. 6811-C.N.R.S. / I.N.P.L., 13 rue du Bois de la Champelle, 54500 Vandœuvre-Lès-Nancy, FRANCE Y1 - 2006 PY - 2006 VL - 25 IS - 2 SP - 1 EP - 10 KW - Torularhodin KW - Carotenoids KW - UV-HPLC / APCI-MS KW - Sporobolmyces ruberrimus H110 KW - identification DO - 10.30492/ijcce.2006.7795 N2 - An on-line UV-HPLC / APCI-MS (atmospheric pressure chemical ionization) tandem by a platinium C18 column with an isocratic solvent system after optimizing was applied to the separation and the identification of different carotenoids including astaxanthin, canthaxanthin, apocarotenoic ester, torularhodin and beta carotene. This method was used to identify particular carotenoids produced by newly isolated microorganism strain of Sporobolomyces ruberrimus H110 in our laboratory. The developed method allows to distinguish torularhodin and canthaxanthin, having the same molar mass but a different chemical structure. A detection limit of 9ng /ml for torularhodin observed. CV (%) for measurement of all carotenoid concentration was always below 3 %. Torularhodin and for a smaller part beta carotene were the two pigments identified in Sporobolmyces ruberrimus H110. UR - https://ijcce.ac.ir/article_7795.html L1 - https://ijcce.ac.ir/article_7795_51f422bed76de1596da2022655c6e6ca.pdf ER -