TY - JOUR ID - 7625 TI - Gum Tragacanth Gels as a New Supporting Matrix for Immobilization of Whole-Cell JO - Iranian Journal of Chemistry and Chemical Engineering JA - IJCCE LA - en SN - 1021-9986 AU - Otady, Maryam AU - Vaziri, Ali AU - Seifkordi, Ali Akbar AU - Kheirolomoom, Azadeh AD - Department of Biotechnology and Chemical Engineering, College of Science and Research, Islamic Azad University, Tehran, I.R. IRAN AD - Department of Chemical Engineering, Sharif University of Technology, Tehran, I.R. IRAN Y1 - 2005 PY - 2005 VL - 24 IS - 4 SP - 1 EP - 7 KW - Gum tragacanth KW - Immobilized whole-cell KW - Penicillin G acylase KW - Ionomer KW - Crosslink DO - 10.30492/ijcce.2005.7625 N2 - We introduce a new smooth, non-toxic, biocompatible method for cross-linking of gum tragacanth (GT), a polysaccharide of natural origin, in order to serve as a new supporting matrix for immobilization systems.  The modified gum is used as a matrix for the catalysis of the conversion of benzyl penicillin to 6-aminopenicillanic acid (6-APA) by means of Escherichia coli ATCC11105 with penicillin G acylase (PGA) activity.  The results show that GT beads can not only serve as a proper matrix for immobilization, but show enhanced hydrolysis rate and stability compared to other immobilization systems used for this reaction.  This signifies the potential of GT as a biocompatible matrix for immobilization and its positive prospects for use in more demanding immobilization applications where traditional matrices such as alginate may fall short. The effect of environmental factors, such as temperature, pH, and substrate concentration, have also been studied on the hydrolysis rate and compared with the other immobilizing systems used for the same reaction, such as calcium alginate.  Under the optimal conditions, penicillin G conversion reached 91.5% after 6 h and remained over 80% after 45 repeated cycles of 6 h each.  UR - https://ijcce.ac.ir/article_7625.html L1 - https://ijcce.ac.ir/article_7625_215fbf4a621829f432c55734f9fbe0fb.pdf ER -