TY - JOUR ID - 7132 TI - Fabrication of a Sulfite Biosensor by the Use of Conducting Polymer JO - Iranian Journal of Chemistry and Chemical Engineering JA - IJCCE LA - en SN - 1021-9986 AU - Hosseini, Mohammad AU - Bahmani, Baharak AU - Moztarzadeh, Fatollah AU - Rabiee, Mohammad AD - Department of Chemical Engineering, Amirkabir University of Technology, P.O. Box 15874-4413 Tehran, I.R. IRAN AD - Department of Biomedical Engineering, Amirkabir University of Technology, P.O. Box 15875-4413 Tehran, I.R. IRAN Y1 - 2008 PY - 2008 VL - 27 IS - 1 SP - 115 EP - 121 KW - Sulfite biosensor KW - Aniline KW - Sulfite oxidase KW - Conducting polymer DO - 10.30492/ijcce.2008.7132 N2 - In this research, an enzyme modified electrode has been produced during the electropolymerization of aniline through incorporation of sulfite oxidase into a conducting polymer. Then the bioelectrochemical response of resulted sulfite biosensor was investigated at different experimental conditions. Study of  the stability of the resulted sulfite biosensor revealed that formation of a passive film on the aluminum surface causes improved stability of the electroactive films formed on the electrode surface. The bioelectrochemical response of the enzyme-modified electrode as a sulfite biosensor was investigated at different experimental conditions. The optimum pH and temperature were 8.5 and 35°C, respectively. The apparent Michaelis-Menten constant and the activation energy of the enzyme catalyzed reaction were calculated. UR - https://ijcce.ac.ir/article_7132.html L1 - https://ijcce.ac.ir/article_7132_d5c60d14f7a56824ba461c2e2ae9756a.pdf ER -