TY - JOUR ID - 36708 TI - Aqueous Two-Phase Systems for the Isolation and Partial Purification of Lipases from Soil Bacteria JO - Iranian Journal of Chemistry and Chemical Engineering JA - IJCCE LA - en SN - 1021-9986 AU - Saddique, Haris AU - Aasim, Muhammad AU - Nawab, Said AU - Bibi, Noor Shad AU - Muhammad, Noor AU - Qasim, Muhammad AD - Department of Biotechnology, University of Malakand, Lower Dir, Khyber Pakhtunkhwa, PAKISTAN AD - Department of Biochemistry, Abdul Wali Khan University Mardan, Khyber Pakhtunkhwa, PAKISTAN AD - Department of Biotechnology and Genetic Engineering, Kohat University of Science and Technology, Kohat, Khyber Pakhtunkhwa, PAKISTAN AD - Department of Microbiology, Kohat University of Science and Technology, Kohat, Khyber Pakhtunkhwa, PAKISTAN Y1 - 2020 PY - 2020 VL - 39 IS - 6 SP - 281 EP - 292 KW - Aqueous two-phase system KW - Polyethylene glycol KW - ammonium sulfate KW - Lipases DO - 10.30492/ijcce.2019.36708 N2 - Aqueous Two-Phase System (ATPS) is an effective, simple, and single-step technique used for the partial purification of biological molecules. The aim of the study was to develop ATPS technique for the isolation and partial purification of lipases. ATPS composed of polyethylene glycol and ammonium sulfate was used in this study to partially purify lipase from the fermentation broth of a Bacillus strain isolated from local soil. The effect of different percent compositions of PEG 4000, 6000, and 10,000 along with 6.60%, 7.26%, 7.92%, and 8.26% ammonium sulfate was studied on the separation behavior of lipase. The optimal condition for the isolation and partial purification of lipases from soil bacterial was 12.5 % of PEG 10,000 with 7.92% ammonium sulfate. The recovery of lipase was 78.3% in the top phase (rich in PEG). UR - https://ijcce.ac.ir/article_36708.html L1 - https://ijcce.ac.ir/article_36708_ca05d84a856e31292654c2f7fe033542.pdf ER -