TY - JOUR ID - 32436 TI - Antibacterial Activity of the Lipopeptide Biosurfactant Produced by Bacillus mojavensis PTCC 1696 JO - Iranian Journal of Chemistry and Chemical Engineering JA - IJCCE LA - en SN - 1021-9986 AU - Ghojavand, Hossein AU - Mohammadi Behnazar, Masoud AU - Vahabzadeh, Farzaneh AD - Administration of Technology Affairs, Deputy of Engineering, Research and Technology, Ministry of Petroleum of Iran, Tehran, I.R. IRAN AD - Department of Chemical Engineering, Amirkabir University of Technology, Tehran, I.R. IRAN Y1 - 2019 PY - 2019 VL - 38 IS - 6 SP - 275 EP - 284 KW - Antibacterial activity KW - Lipopeptide, biosurfactant KW - Surface activity KW - Bacillus mojavensis PTCC 1696 DO - 10.30492/ijcce.2019.32436 N2 - Bacillus mojavensis PTCC 1696 (a member of Bacillus subtilis group) has been isolated from the Iranian oil field (Masjed-I Soleyman), to examine its ability to produce biosurfactant (lipopeptide type) [28]. The present study was designed to characterize the antibacterial activity of the isolated biosurfactant. The antibacterial activity towards several bacteria including clinical isolates and type strains species was examined. For detecting the extent of antibacterial activity, the agar disc diffusion method was used, where the measured diameter of the zone of inhibition was used as an index for determining the antibacterial activity. Among the test microorganisms, the antibacterial activity was highest in Pseudomonas aeruginosa ATCC 27853. At concentration as low as 16 μg/ml, the inhibitory effect of the lipopeptide biosurfactant was detectable. The stability of the test biosurfactant also was examined over a wide range of temperatures (40–100°C)and pH values (2-11). The stability was further tested using protease and lipase, where the substance showed clear sensitivity towards lipase. The potentiality of this antibacterial agent in clinical applications is of interest and needs to be further recognized. UR - https://ijcce.ac.ir/article_32436.html L1 - https://ijcce.ac.ir/article_32436_16f4c802b4fc74d0900c2cb75c8c9ba4.pdf ER -