TY - JOUR ID - 28069 TI - Solid Phase Extraction Using Modified Magnetic Iron Oxide Nanoparticles for Extraction and Spectrofluorimetric Determination of Carvedilol in Human Plasma Samples JO - Iranian Journal of Chemistry and Chemical Engineering JA - IJCCE LA - en SN - 1021-9986 AU - Bavili Tabrizi, Ahad AU - Panahi, Mahdi AD - Biotechnology Research Center, Tabriz University of Medical Sciences, Tabriz, I.R. IRAN+/Department of Medicinal Chemistry, Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz. I.R. IRAN AD - Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, I.R. IRAN Y1 - 2017 PY - 2017 VL - 36 IS - 3 SP - 115 EP - 125 KW - Carvedilol KW - Magnetic iron oxide nanoparticles KW - Plasma KW - Solid phase extraction KW - Spectrofluorimetry DO - 10.30492/ijcce.2017.28069 N2 - A new analytical approach was developed involving magnetic solid–phase extraction and spectrofluorimetric determination of carvedilol in human plasma samples. A plasma sample was prepared and adjusted to pH 8.2–10, then carvedilol was quickly extracted using iron oxide magnetic nanoparticles modified by the surfactant cetyltrimethylammonium bromide and determined to apply spectrofluorimetry at 354 ± 3 nm after excitation at 241 ± 3 nm. Experimental conditions, such as the amount of nanoparticles and cetyltrimethylammonium bromide, pH value, standing time and desorption solvent type and volume have been adjusted to optimize the extraction process and to obtain analytical characteristics of the method. Linearity was observed in the analyte concentration range of 2.0–125 ng/mL with correlation coefficients (r) of 0.999. The method showed good precision and accuracy, with intra– and inter–assay precisions of less than 7.0% at all concentrations. Standard addition recovery tests were carried out, and the recoveries ranged from 94.4% to 100.7%. The limits of detection and quantification were found to be 0.67 and 2.24 ng/mL. The method was applied to the determination of carvedilol in human plasma samples.  UR - https://ijcce.ac.ir/article_28069.html L1 - https://ijcce.ac.ir/article_28069_1066266fb6c8021ee9321dca83efd491.pdf ER -