TY - JOUR ID - 10067 TI - Measurement of Michaelis Constants for ATP and Mg2+ in Bioluminescence Reaction of Luciferase by a Home-Made Luminometer JO - Iranian Journal of Chemistry and Chemical Engineering JA - IJCCE LA - en SN - 1021-9986 AU - Hadjmohammadi, Mohammad Reza AU - Chaichi, Mohammad Javad AD - Institute of Chemistry, Mazandaran University, P.O. Box 311, Babolsar, I.R. IRAN Y1 - 1999 PY - 1999 VL - 18 IS - 2 SP - 83 EP - 87 KW - Michaelis constant KW - luciferase KW - ATP KW - Photinus pyralis KW - American firefly KW - Luminometer KW - Bioluminescence DO - 10.30492/ijcce.1999.10067 N2 - Effects of ATP and Mg2+ concentrations on bioluminescence reaction of luciferase (Photinus pyralis) were investigated by home-made luminometer. The Michaelis constants of the enzyme for ATP and Mg2+ obtained from the Lineweaver-Burk graph, were 61.9 mM ±3.3 mM and 251.6mM ± 39.0mM respectively. Optimum concentration of Mg2+ for maximum luminescence was 0.004 M. UR - https://ijcce.ac.ir/article_10067.html L1 - https://ijcce.ac.ir/article_10067_f529ca345f80b9ec23bc1c2bff7eefb8.pdf ER -