%0 Journal Article %T Separation of Biojenic Amines Using Dansyl Cloride Derivatization and Mixed Micellar Liquid Chromatography %J Iranian Journal of Chemistry and Chemical Engineering %I Iranian Institute of Research and Development in Chemical Industries (IRDCI)-ACECR %Z 1021-9986 %A Hadjmohammadi, Mohammad Reza %A Jafari, Soghra %D 2006 %\ 03/01/2006 %V 25 %N 1 %P 19-23 %! Separation of Biojenic Amines Using Dansyl Cloride Derivatization and Mixed Micellar Liquid Chromatography %K Biogenic amines %K HPLC %K Micellar liquid chromatography %K Mixed micelles %R 10.30492/ijcce.2006.8094 %X Separation of some biogenic amines via RP-HPLC using mixed micellar mobile phase was investigated. The compounds were derivatized before hand by dansyl chloride as a chromophoric reagent. Appropriate conditions for separation, were determined by studying factors such as temperature, type and percentage of organic modifier, concentration of surfactants (SDS and Brij-35) and the pH of the mobile phase. The number of theoretical plates (N), asymmetry factor (B/A) and selectivity factor (α) considered as criteria for finding the appropriate conditions for separation. Appropriate condition, were: 40˚C, 40 mM SDS, 0.5 mM Brij-35 and 10% 1-propanol at pH=5. %U https://ijcce.ac.ir/article_8094_8db9366f82bdde0254e41dee7e0e373b.pdf