%0 Journal Article %T Decolorization of Maxilon-Red by Kissiris Immobilized Phanerochaete Chrysosporium in a Trickle-Bed Bioreactor-Involvement of Ligninolytic Enzymes %J Iranian Journal of Chemistry and Chemical Engineering %I Iranian Institute of Research and Development in Chemical Industries (IRDCI)-ACECR %Z 1021-9986 %A Karimi, Afzal %A Vahabzadeh, Farzaneh %A Mohseni, Majid %A Mehranian, Mehrnaz %D 2009 %\ 06/01/2009 %V 28 %N 2 %P 1-13 %! Decolorization of Maxilon-Red by Kissiris Immobilized Phanerochaete Chrysosporium in a Trickle-Bed Bioreactor-Involvement of Ligninolytic Enzymes %K Ligninolytic enzymes %K Kissiris-immobilized Phanerochaete chrysosporium %K Decolorization %K Maxilon-red synthetic dye %K Trickle-bed bioreactor %R 10.30492/ijcce.2009.6854 %X The decolorization of Maxilon-red dye by Kissiris immobilized Phanerochaete chrysosporium in the trickle-bed reactor (TBR) using the basal nitrogen-limited growth medium was studied. The influence of the superficial liquid velocity (SLV) on the decolorizing ability of the fungus was examined at four SLVs (cm sec-1): 0.05, 0.075, 0.1, and 0.15. Maximum level of the decolorizing activity was about 94 % by day 4-5 of the process when the TBR operated at SLV=0.075  to  0.1 cm sec-1. At these SLVs, the maximum activities of the lignin peroxidase (LiP) and the manganese peroxidase (MnP) were (UL-1): 87 and 207, respectively. In a successive decolorization of the tested dye in a continuous dye addition in the TBR when glucose level decreased to 2 gL-1, significant reduction in the characteristics of the interest were observed. While by controlling the glucose concentration, these properties were improved considerably: The decolorizing ability of the Kissiris immobilized mycelia was more than 85 % after nearly 20 days of the process while the maximum activities of the LiP and the MnP were (UL-1): 320 and 151, respectively. Considerable levels of the enzyme activities were detected by day 19 and after 11 times of the dye additions. %U https://ijcce.ac.ir/article_6854_b0145843c7641ad1105c181a281d3bcb.pdf