%0 Journal Article %T Measurement of Michaelis Constants for ATP and Mg2+ in Bioluminescence Reaction of Luciferase by a Home-Made Luminometer %J Iranian Journal of Chemistry and Chemical Engineering %I Iranian Institute of Research and Development in Chemical Industries (IRDCI)-ACECR %Z 1021-9986 %A Hadjmohammadi, Mohammad Reza %A Chaichi, Mohammad Javad %D 1999 %\ 11/01/1999 %V 18 %N 2 %P 83-87 %! Measurement of Michaelis Constants for ATP and Mg2+ in Bioluminescence Reaction of Luciferase by a Home-Made Luminometer %K Michaelis constant %K luciferase %K ATP %K Photinus pyralis %K American firefly %K Luminometer %K Bioluminescence %R 10.30492/ijcce.1999.10067 %X Effects of ATP and Mg2+ concentrations on bioluminescence reaction of luciferase (Photinus pyralis) were investigated by home-made luminometer. The Michaelis constants of the enzyme for ATP and Mg2+ obtained from the Lineweaver-Burk graph, were 61.9 mM ±3.3 mM and 251.6mM ± 39.0mM respectively. Optimum concentration of Mg2+ for maximum luminescence was 0.004 M. %U https://ijcce.ac.ir/article_10067_f529ca345f80b9ec23bc1c2bff7eefb8.pdf